12 Gao BR 46-2.indd
نویسندگان
چکیده
The fresh-water green unicellular alga Haematococcus pluvialis is known to accumulate astaxanthin under stress conditions. In the present study, transcriptional expression of eight genes involved in astaxanthin biosynthesis exposed to EBR (25 and 50 mg/L) was analyzed using qRT-PCR. The results demonstrated that both 25 and 50 mg/L EBR could increase astaxanthin productivity and the eight carotenogenic genes were up-regulated by EBR with diff erent expression profi les. Moreover, EBR25 induction had a greater infl uence on the transcriptional expression of ipi-1, ipi-2, crtR-B, lyc and crtO (> 5fold up-regulation) than on psy, pds, bkt; EBR50 treatment had a greater eff ect on the transcriptional expression of ipi-2, pds, lyc, crtR-B, bkt and crtO than on ipi-1 and psy. Furthermore, astaxanthin biosynthesis under EBR was up-regulated mainly by ipi-1 and psy at the post-transcriptional level, pds, lyc, crtR-B, bkt and crtO at the transcriptional level and ipi-2 at both levels. Key terms: Haematococcus pluvialis, astaxanthin, 2, 4-Epibrassinolide (EBR), carotenoid genes, real-time fl uorescence quantitative PCR (qRTPCR). Abbreviations: SA: salicylic acid; JA: jasmonic acid; BRs: Brassinosteroids; EBR: 2, 4-Epibrassinolide
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